The tea (Camellia sinensis L.)  plantation is an integrated ecosystem combining conservation and utilization of natural resources. This study aims to propose innovative approach to develop a simple and time efficient to assess and monitor biodiversity of tea plantation in Java island using Unmanned Aerial Vehicle (UAV), popularly known as drone. In this study, quadcopter drone was flown to conduct aerial surveys and take imagery of ground sample plots measuring 20 m×20 m. Moreover, various maps of tea plantation biodiversity were developed using drone imagery and overlaid with data collected from ground surveys. As a result, the drone based imagery of a tea field always varies with respect to the life forms of plant species and these could be well monitored and measured using drone multispectral sensors. The drone imagery has succeeded to detect the plant diversity in tea fields that slightly lower than in the intact forest fields. Based on the drone imagery, the life forms of plant species in tea fields are as follows grasses> shrubs>arbors. In contrast, there are more arbors and shrubs in the forest fields. Moreover, information from remotely sensed drone can be integrated with plant species data which can provide insights to the cultural practices being implied into the tea plantation. It will also help tea farmers identify conservation and utilization areas within a tea plantation. Hence, using UAV as an assessment tool, tea farmers will not only improve the productivity of their land, but will also minimize environmental impacts.